Characterization of a fibronectin‐binding protein from Lactobacillus casei BL23

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Characterization of a fibronectin-binding protein from Lactobacillus casei BL23.

AIMS To characterize the functionality of the Lactobacillus casei BL23 fbpA gene encoding a putative fibronectin-binding protein. METHODS AND RESULTS Adhesion tests showed that L. casei BL23 binds immobilized and soluble fibronectin in a protease-sensitive manner. A mutant with inactivated fbpA showed a decrease in binding to immobilized fibronectin and a strong reduction in the surface hydro...

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Sortases are a class of enzymes that anchor surface proteins to the cell wall of Gram-positive bacteria. Lactobacillus casei BL23 harbors four sortase genes, two belonging to class A (srtA1 and srtA2) and two belonging to class C (srtC1 and srtC2). Class C sortases were clustered with genes encoding their putative substrates that were homologous to the SpaEFG and SpaCBA proteins that encode muc...

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ژورنال

عنوان ژورنال: Journal of Applied Microbiology

سال: 2010

ISSN: 1364-5072,1365-2672

DOI: 10.1111/j.1365-2672.2009.04508.x